c c6 36 cells Search Results


c6/36 cells  (Corning Life Sciences)
90
Corning Life Sciences c6/36 cells
Detection of DEN-1 in <t> C6/36 cells </t> infected at different MOIs on day 1 postinoculation using indirect IF stain
C6/36 Cells, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c6/36 jcrb strain  (JCRB Cell Bank)
90
JCRB Cell Bank c6/36 jcrb strain
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
C6/36 Jcrb Strain, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c6/36 cells  (BEI Resources)
90
BEI Resources c6/36 cells
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
C6/36 Cells, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nih/3t3 cell  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection nih/3t3 cell
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
Nih/3t3 Cell, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c6/36 cells  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures c6/36 cells
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
C6/36 Cells, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+c6+36+cells/c6+36+cells/pm36922882-61-12-14
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c6/36 cells bcrc-60114  (BioResource International Inc)
90
BioResource International Inc c6/36 cells bcrc-60114
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
C6/36 Cells Bcrc 60114, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c6/36 cells  (HiMedia Laboratories)
90
HiMedia Laboratories c6/36 cells
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
C6/36 Cells, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+c6+36+cells/c6+36+cells/pm30472865-52-0-13
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c6/36 cell-derived denv-2  (MBL Life science)
90
MBL Life science c6/36 cell-derived denv-2
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
C6/36 Cell Derived Denv 2, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+c6+36+cells/binding+to+c6+36+cell+derived+denv+2/10__1128_slash_mbio__00276___11-114-2-8
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aedes albopictus cell line c6/36  (National Centre for Cell Science)
90
National Centre for Cell Science aedes albopictus cell line c6/36
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
Aedes Albopictus Cell Line C6/36, supplied by National Centre for Cell Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+c6+36+cells/aedes+albopictus+cell+line+c6+36/pm15105530-23-15-4
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aedes albopictus derived c6/36 cells  (rocky mountain labs)
90
rocky mountain labs aedes albopictus derived c6/36 cells
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
Aedes Albopictus Derived C6/36 Cells, supplied by rocky mountain labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aedes albopictus midgut c6/36 cells  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare aedes albopictus midgut c6/36 cells
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
Aedes Albopictus Midgut C6/36 Cells, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aedes albopictus c6/36 cells  (Vitrocell Systems GmbH)
90
Vitrocell Systems GmbH aedes albopictus c6/36 cells
Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 <t>JCRB</t> strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
Aedes Albopictus C6/36 Cells, supplied by Vitrocell Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Detection of DEN-1 in C6/36 cells infected at different MOIs on day 1 postinoculation using indirect IF stain

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Detection of DEN-1 in C6/36 cells infected at different MOIs on day 1 postinoculation using indirect IF stain

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Infection

Comparison of three fixation-permeabilization methods for the detection of dengue virus in C6/36 cells using flow cytometry a

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of three fixation-permeabilization methods for the detection of dengue virus in C6/36 cells using flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Flow Cytometry

Comparison of three MAbs used for the detection of DEN-1 virus (Hawaii) antigen in C6/36 cells using flow cytometry a

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of three MAbs used for the detection of DEN-1 virus (Hawaii) antigen in C6/36 cells using flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Flow Cytometry

Flow cytometric histograms of DEN-1 (Hawaii strain)-infected C6/36 cells stained with DEN-1 MAb 16-4 (anti-NS1) (black) overlaid with histograms of mock-infected cells (white). C6/36 mosquito cells were infected with DEN-1 (Hawaii) at an MOI of 0.01. (A through G) Different time points postinfection. FL1-H, FITC fluorescence intensity. The bars represent the proportion of positive cells. (H) Detection of DEN-1 viral RNA in infected C6/36 cells collected at different time points postinfection using RT-nPCR. Lanes 1 to 8 show results at 8, 12, 16, 24, 26, 30, 48, and 72 h postinfection, respectively. Lane 9, mock-infected C6/36 cells; lane 10, culture medium only; lane 11, DEN-1 (Hawaii) virus; lane 12, reagent control; lane M, molecular size markers.

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Flow cytometric histograms of DEN-1 (Hawaii strain)-infected C6/36 cells stained with DEN-1 MAb 16-4 (anti-NS1) (black) overlaid with histograms of mock-infected cells (white). C6/36 mosquito cells were infected with DEN-1 (Hawaii) at an MOI of 0.01. (A through G) Different time points postinfection. FL1-H, FITC fluorescence intensity. The bars represent the proportion of positive cells. (H) Detection of DEN-1 viral RNA in infected C6/36 cells collected at different time points postinfection using RT-nPCR. Lanes 1 to 8 show results at 8, 12, 16, 24, 26, 30, 48, and 72 h postinfection, respectively. Lane 9, mock-infected C6/36 cells; lane 10, culture medium only; lane 11, DEN-1 (Hawaii) virus; lane 12, reagent control; lane M, molecular size markers.

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Infection, Staining, Fluorescence

Comparison of the sensitivity in detection of DEN-1 virus (Hawaii)-infected C6/36 cells by conventional IF staining and flow cytometry a

Journal:

Article Title: Flow Cytometry Compared with Indirect Immunofluorescence for Rapid Detection of Dengue Virus Type 1 after Amplification in Tissue Culture

doi: 10.1128/JCM.39.10.3672-3677.2001

Figure Lengend Snippet: Comparison of the sensitivity in detection of DEN-1 virus (Hawaii)-infected C6/36 cells by conventional IF staining and flow cytometry a

Article Snippet: After confluent growth of C6/36 cells in 96-well plates (Costar), DEN-1 (Hawaii strain) and two local DEN-1 isolates (766733 and 066267) were inoculated and incubated at 28°C.

Techniques: Staining, Flow Cytometry

Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 JCRB strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.

Journal: Heliyon

Article Title: Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

doi: 10.1016/j.heliyon.2018.e00736

Figure Lengend Snippet: Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 JCRB strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.

Article Snippet: The growth kinetics of flaviviruses in C6/36 JCRB strain was significantly lower than that in C6/36 ECACC strain ( A).

Techniques: Infection, Cell Culture, Genomic Sequencing, Reverse Transcription Polymerase Chain Reaction, Amplification

C6/36 cells of JCRB and ECACC strains showed different susceptibility to flavivirus infection. Growth kinetics of arboviruses in C6/36 cell lines. A : C6/36 cells of either JCRB or ECACC strain were infected with Zika virus (ZIKV), Japanese encephalitis virus (JEV), or Dengue virus (DENV) at a multiplicity of infection (MOI) of 0.01, and the titers in the supernatants were determined by plaque assay. B : C6/36 cells of either JCRB or ECACC strain were infected with Getah virus (GETV) or Sindbis virus (SINV) with MOI = 0.001. Red lines show the titer in C6/36 JCRB strain and black lines show that in C6/36 ECACC strain. The experiment was triplicated, and the standard deviations are also indicated. Asterisks showed the results of t -test for the data group at each time points (* p < 0.05, ** p < 0.01).

Journal: Heliyon

Article Title: Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses

doi: 10.1016/j.heliyon.2018.e00736

Figure Lengend Snippet: C6/36 cells of JCRB and ECACC strains showed different susceptibility to flavivirus infection. Growth kinetics of arboviruses in C6/36 cell lines. A : C6/36 cells of either JCRB or ECACC strain were infected with Zika virus (ZIKV), Japanese encephalitis virus (JEV), or Dengue virus (DENV) at a multiplicity of infection (MOI) of 0.01, and the titers in the supernatants were determined by plaque assay. B : C6/36 cells of either JCRB or ECACC strain were infected with Getah virus (GETV) or Sindbis virus (SINV) with MOI = 0.001. Red lines show the titer in C6/36 JCRB strain and black lines show that in C6/36 ECACC strain. The experiment was triplicated, and the standard deviations are also indicated. Asterisks showed the results of t -test for the data group at each time points (* p < 0.05, ** p < 0.01).

Article Snippet: The growth kinetics of flaviviruses in C6/36 JCRB strain was significantly lower than that in C6/36 ECACC strain ( A).

Techniques: Infection, Virus, Plaque Assay